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Hypothesis Testing | A Step-by-Step Guide with Easy Examples

Published on November 8, 2019 by Rebecca Bevans . Revised on June 22, 2023.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics . It is most often used by scientists to test specific predictions, called hypotheses, that arise from theories.

There are 5 main steps in hypothesis testing:

• State your research hypothesis as a null hypothesis and alternate hypothesis (H o ) and (H a  or H 1 ).
• Collect data in a way designed to test the hypothesis.
• Perform an appropriate statistical test .
• Decide whether to reject or fail to reject your null hypothesis.
• Present the findings in your results and discussion section.

Though the specific details might vary, the procedure you will use when testing a hypothesis will always follow some version of these steps.

Table of contents

Step 1: state your null and alternate hypothesis, step 2: collect data, step 3: perform a statistical test, step 4: decide whether to reject or fail to reject your null hypothesis, step 5: present your findings, other interesting articles, frequently asked questions about hypothesis testing.

After developing your initial research hypothesis (the prediction that you want to investigate), it is important to restate it as a null (H o ) and alternate (H a ) hypothesis so that you can test it mathematically.

The alternate hypothesis is usually your initial hypothesis that predicts a relationship between variables. The null hypothesis is a prediction of no relationship between the variables you are interested in.

• H 0 : Men are, on average, not taller than women. H a : Men are, on average, taller than women.

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For a statistical test to be valid , it is important to perform sampling and collect data in a way that is designed to test your hypothesis. If your data are not representative, then you cannot make statistical inferences about the population you are interested in.

There are a variety of statistical tests available, but they are all based on the comparison of within-group variance (how spread out the data is within a category) versus between-group variance (how different the categories are from one another).

If the between-group variance is large enough that there is little or no overlap between groups, then your statistical test will reflect that by showing a low p -value . This means it is unlikely that the differences between these groups came about by chance.

Alternatively, if there is high within-group variance and low between-group variance, then your statistical test will reflect that with a high p -value. This means it is likely that any difference you measure between groups is due to chance.

Your choice of statistical test will be based on the type of variables and the level of measurement of your collected data .

• an estimate of the difference in average height between the two groups.
• a p -value showing how likely you are to see this difference if the null hypothesis of no difference is true.

Based on the outcome of your statistical test, you will have to decide whether to reject or fail to reject your null hypothesis.

In most cases you will use the p -value generated by your statistical test to guide your decision. And in most cases, your predetermined level of significance for rejecting the null hypothesis will be 0.05 – that is, when there is a less than 5% chance that you would see these results if the null hypothesis were true.

In some cases, researchers choose a more conservative level of significance, such as 0.01 (1%). This minimizes the risk of incorrectly rejecting the null hypothesis ( Type I error ).

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The results of hypothesis testing will be presented in the results and discussion sections of your research paper , dissertation or thesis .

In the results section you should give a brief summary of the data and a summary of the results of your statistical test (for example, the estimated difference between group means and associated p -value). In the discussion , you can discuss whether your initial hypothesis was supported by your results or not.

In the formal language of hypothesis testing, we talk about rejecting or failing to reject the null hypothesis. You will probably be asked to do this in your statistics assignments.

However, when presenting research results in academic papers we rarely talk this way. Instead, we go back to our alternate hypothesis (in this case, the hypothesis that men are on average taller than women) and state whether the result of our test did or did not support the alternate hypothesis.

If your null hypothesis was rejected, this result is interpreted as “supported the alternate hypothesis.”

These are superficial differences; you can see that they mean the same thing.

You might notice that we don’t say that we reject or fail to reject the alternate hypothesis . This is because hypothesis testing is not designed to prove or disprove anything. It is only designed to test whether a pattern we measure could have arisen spuriously, or by chance.

If we reject the null hypothesis based on our research (i.e., we find that it is unlikely that the pattern arose by chance), then we can say our test lends support to our hypothesis . But if the pattern does not pass our decision rule, meaning that it could have arisen by chance, then we say the test is inconsistent with our hypothesis .

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Normal distribution
• Descriptive statistics
• Measures of central tendency
• Correlation coefficient

Methodology

• Cluster sampling
• Stratified sampling
• Types of interviews
• Cohort study
• Thematic analysis

Research bias

• Implicit bias
• Cognitive bias
• Survivorship bias
• Availability heuristic
• Nonresponse bias
• Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

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Understanding Science

How science REALLY works...

• Understanding Science 101
• Misconceptions
• Testing ideas with evidence is at the heart of the process of science.
• Scientific testing involves figuring out what we would  expect  to observe if an idea were correct and comparing that expectation to what we  actually  observe.

Misconception:  Science proves ideas.

Misconception:  Science can only disprove ideas.

Correction:  Science neither proves nor disproves. It accepts or rejects ideas based on supporting and refuting evidence, but may revise those conclusions if warranted by new evidence or perspectives.  Read more about it.

Testing scientific ideas

Testing ideas about childbed fever.

As a simple example of how scientific testing works, consider the case of Ignaz Semmelweis, who worked as a doctor on a maternity ward in the 1800s. In his ward, an unusually high percentage of new mothers died of what was then called childbed fever. Semmelweis considered many possible explanations for this high death rate. Two of the many ideas that he considered were (1) that the fever was caused by mothers giving birth lying on their backs (as opposed to on their sides) and (2) that the fever was caused by doctors’ unclean hands (the doctors often performed autopsies immediately before examining women in labor). He tested these ideas by considering what expectations each idea generated. If it were true that childbed fever were caused by giving birth on one’s back, then changing procedures so that women labored on their sides should lead to lower rates of childbed fever. Semmelweis tried changing the position of labor, but the incidence of fever did not decrease; the actual observations did not match the expected results. If, however, childbed fever were caused by doctors’ unclean hands, having doctors wash their hands thoroughly with a strong disinfecting agent before attending to women in labor should lead to lower rates of childbed fever. When Semmelweis tried this, rates of fever plummeted; the actual observations matched the expected results, supporting the second explanation.

Testing in the tropics

Let’s take a look at another, very different, example of scientific testing: investigating the origins of coral atolls in the tropics. Consider the atoll Eniwetok (Anewetak) in the Marshall Islands — an oceanic ring of exposed coral surrounding a central lagoon. From the 1800s up until today, scientists have been trying to learn what supports atoll structures beneath the water’s surface and exactly how atolls form. Coral only grows near the surface of the ocean where light penetrates, so Eniwetok could have formed in several ways:

Hypothesis 2: The coral that makes up Eniwetok might have grown in a ring atop an underwater mountain already near the surface. The key to this hypothesis is the idea that underwater mountains don’t sink; instead the remains of dead sea animals (shells, etc.) accumulate on underwater mountains, potentially assisted by tectonic uplifting. Eventually, the top of the mountain/debris pile would reach the depth at which coral grow, and the atoll would form.

Which is a better explanation for Eniwetok? Did the atoll grow atop a sinking volcano, forming an underwater coral tower, or was the mountain instead built up until it neared the surface where coral were eventually able to grow? Which of these explanations is best supported by the evidence? We can’t perform an experiment to find out. Instead, we must figure out what expectations each hypothesis generates, and then collect data from the world to see whether our observations are a better match with one of the two ideas.

If Eniwetok grew atop an underwater mountain, then we would expect the atoll to be made up of a relatively thin layer of coral on top of limestone or basalt. But if it grew upwards around a subsiding island, then we would expect the atoll to be made up of many hundreds of feet of coral on top of volcanic rock. When geologists drilled into Eniwetok in 1951 as part of a survey preparing for nuclear weapons tests, the drill bored through more than 4000 feet (1219 meters) of coral before hitting volcanic basalt! The actual observation contradicted the underwater mountain explanation and matched the subsiding island explanation, supporting that idea. Of course, many other lines of evidence also shed light on the origins of coral atolls, but the surprising depth of coral on Eniwetok was particularly convincing to many geologists.

• Take a sidetrip

Visit the NOAA website to see an animation of coral atoll formation according to Hypothesis 1.

• Teaching resources

Scientists test hypotheses and theories. They are both scientific explanations for what we observe in the natural world, but theories deal with a much wider range of phenomena than do hypotheses. To learn more about the differences between hypotheses and theories, jump ahead to  Science at multiple levels .

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S.3 hypothesis testing.

In reviewing hypothesis tests, we start first with the general idea. Then, we keep returning to the basic procedures of hypothesis testing, each time adding a little more detail.

The general idea of hypothesis testing involves:

• Making an initial assumption.
• Collecting evidence (data).
• Based on the available evidence (data), deciding whether to reject or not reject the initial assumption.

Every hypothesis test — regardless of the population parameter involved — requires the above three steps.

Example S.3.1

Is normal body temperature really 98.6 degrees f section  .

Consider the population of many, many adults. A researcher hypothesized that the average adult body temperature is lower than the often-advertised 98.6 degrees F. That is, the researcher wants an answer to the question: "Is the average adult body temperature 98.6 degrees? Or is it lower?" To answer his research question, the researcher starts by assuming that the average adult body temperature was 98.6 degrees F.

Then, the researcher went out and tried to find evidence that refutes his initial assumption. In doing so, he selects a random sample of 130 adults. The average body temperature of the 130 sampled adults is 98.25 degrees.

Then, the researcher uses the data he collected to make a decision about his initial assumption. It is either likely or unlikely that the researcher would collect the evidence he did given his initial assumption that the average adult body temperature is 98.6 degrees:

• If it is likely , then the researcher does not reject his initial assumption that the average adult body temperature is 98.6 degrees. There is not enough evidence to do otherwise.
• either the researcher's initial assumption is correct and he experienced a very unusual event;
• or the researcher's initial assumption is incorrect.

In statistics, we generally don't make claims that require us to believe that a very unusual event happened. That is, in the practice of statistics, if the evidence (data) we collected is unlikely in light of the initial assumption, then we reject our initial assumption.

Example S.3.2

Criminal trial analogy section  .

One place where you can consistently see the general idea of hypothesis testing in action is in criminal trials held in the United States. Our criminal justice system assumes "the defendant is innocent until proven guilty." That is, our initial assumption is that the defendant is innocent.

In the practice of statistics, we make our initial assumption when we state our two competing hypotheses -- the null hypothesis ( H 0 ) and the alternative hypothesis ( H A ). Here, our hypotheses are:

• H 0 : Defendant is not guilty (innocent)
• H A : Defendant is guilty

In statistics, we always assume the null hypothesis is true . That is, the null hypothesis is always our initial assumption.

The prosecution team then collects evidence — such as finger prints, blood spots, hair samples, carpet fibers, shoe prints, ransom notes, and handwriting samples — with the hopes of finding "sufficient evidence" to make the assumption of innocence refutable.

In statistics, the data are the evidence.

The jury then makes a decision based on the available evidence:

• If the jury finds sufficient evidence — beyond a reasonable doubt — to make the assumption of innocence refutable, the jury rejects the null hypothesis and deems the defendant guilty. We behave as if the defendant is guilty.
• If there is insufficient evidence, then the jury does not reject the null hypothesis . We behave as if the defendant is innocent.

In statistics, we always make one of two decisions. We either "reject the null hypothesis" or we "fail to reject the null hypothesis."

Errors in Hypothesis Testing Section  

Did you notice the use of the phrase "behave as if" in the previous discussion? We "behave as if" the defendant is guilty; we do not "prove" that the defendant is guilty. And, we "behave as if" the defendant is innocent; we do not "prove" that the defendant is innocent.

This is a very important distinction! We make our decision based on evidence not on 100% guaranteed proof. Again:

• If we reject the null hypothesis, we do not prove that the alternative hypothesis is true.
• If we do not reject the null hypothesis, we do not prove that the null hypothesis is true.

We merely state that there is enough evidence to behave one way or the other. This is always true in statistics! Because of this, whatever the decision, there is always a chance that we made an error .

Let's review the two types of errors that can be made in criminal trials:

Table S.3.2 shows how this corresponds to the two types of errors in hypothesis testing.

Note that, in statistics, we call the two types of errors by two different  names -- one is called a "Type I error," and the other is called  a "Type II error." Here are the formal definitions of the two types of errors:

There is always a chance of making one of these errors. But, a good scientific study will minimize the chance of doing so!

Making the Decision Section  

Recall that it is either likely or unlikely that we would observe the evidence we did given our initial assumption. If it is likely , we do not reject the null hypothesis. If it is unlikely , then we reject the null hypothesis in favor of the alternative hypothesis. Effectively, then, making the decision reduces to determining "likely" or "unlikely."

In statistics, there are two ways to determine whether the evidence is likely or unlikely given the initial assumption:

• We could take the " critical value approach " (favored in many of the older textbooks).
• Or, we could take the " P -value approach " (what is used most often in research, journal articles, and statistical software).

In the next two sections, we review the procedures behind each of these two approaches. To make our review concrete, let's imagine that μ is the average grade point average of all American students who major in mathematics. We first review the critical value approach for conducting each of the following three hypothesis tests about the population mean $\mu$:

In Practice

• We would want to conduct the first hypothesis test if we were interested in concluding that the average grade point average of the group is more than 3.
• We would want to conduct the second hypothesis test if we were interested in concluding that the average grade point average of the group is less than 3.
• And, we would want to conduct the third hypothesis test if we were only interested in concluding that the average grade point average of the group differs from 3 (without caring whether it is more or less than 3).

Upon completing the review of the critical value approach, we review the P -value approach for conducting each of the above three hypothesis tests about the population mean \(\mu\). The procedures that we review here for both approaches easily extend to hypothesis tests about any other population parameter.

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7.1: Basics of Hypothesis Testing

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• Page ID 16360

• Kathryn Kozak
• Coconino Community College

To understand the process of a hypothesis tests, you need to first have an understanding of what a hypothesis is, which is an educated guess about a parameter. Once you have the hypothesis, you collect data and use the data to make a determination to see if there is enough evidence to show that the hypothesis is true. However, in hypothesis testing you actually assume something else is true, and then you look at your data to see how likely it is to get an event that your data demonstrates with that assumption. If the event is very unusual, then you might think that your assumption is actually false. If you are able to say this assumption is false, then your hypothesis must be true. This is known as a proof by contradiction. You assume the opposite of your hypothesis is true and show that it can’t be true. If this happens, then your hypothesis must be true. All hypothesis tests go through the same process. Once you have the process down, then the concept is much easier. It is easier to see the process by looking at an example. Concepts that are needed will be detailed in this example.

Example \(\PageIndex{1}\) basics of hypothesis testing

Suppose a manufacturer of the XJ35 battery claims the mean life of the battery is 500 days with a standard deviation of 25 days. You are the buyer of this battery and you think this claim is inflated. You would like to test your belief because without a good reason you can’t get out of your contract.

What do you do?

Well first, you should know what you are trying to measure. Define the random variable.

Let x = life of a XJ35 battery

Now you are not just trying to find different x values. You are trying to find what the true mean is. Since you are trying to find it, it must be unknown. You don’t think it is 500 days. If you did, you wouldn’t be doing any testing. The true mean, \(\mu\), is unknown. That means you should define that too.

Let \(\mu\)= mean life of a XJ35 battery

You may want to collect a sample. What kind of sample?

You could ask the manufacturers to give you batteries, but there is a chance that there could be some bias in the batteries they pick. To reduce the chance of bias, it is best to take a random sample.

How big should the sample be?

A sample of size 30 or more means that you can use the central limit theorem. Pick a sample of size 30.

Example \(\PageIndex{1}\) contains the data for the sample you collected:

Now what should you do? Looking at the data set, you see some of the times are above 500 and some are below. But looking at all of the numbers is too difficult. It might be helpful to calculate the mean for this sample.

The sample mean is \(\overline{x} = 490\) days. Looking at the sample mean, one might think that you are right. However, the standard deviation and the sample size also plays a role, so maybe you are wrong.

Before going any farther, it is time to formalize a few definitions.

You have a guess that the mean life of a battery is less than 500 days. This is opposed to what the manufacturer claims. There really are two hypotheses, which are just guesses here – the one that the manufacturer claims and the one that you believe. It is helpful to have names for them.

Definition \(\PageIndex{1}\)

Null Hypothesis : historical value, claim, or product specification. The symbol used is \(H_{o}\).

Definition \(\PageIndex{2}\)

Alternate Hypothesis : what you want to prove. This is what you want to accept as true when you reject the null hypothesis. There are two symbols that are commonly used for the alternative hypothesis: \(H_{A}\) or \(H_{I}\). The symbol \(H_{A}\) will be used in this book.

In general, the hypotheses look something like this:

\(H_{o} : \mu=\mu_{o}\)

\(H_{A} : \mu<\mu_{o}\)

where \(\mu_{o}\) just represents the value that the claim says the population mean is actually equal to.

Also, \(H_{A}\) can be less than, greater than, or not equal to.

For this problem:

\(H_{o} : \mu=500\) days, since the manufacturer says the mean life of a battery is 500 days.

\(H_{A} : \mu<500\) days, since you believe that the mean life of the battery is less than 500 days.

Now back to the mean. You have a sample mean of 490 days. Is this small enough to believe that you are right and the manufacturer is wrong? How small does it have to be?

If you calculated a sample mean of 235, you would definitely believe the population mean is less than 500. But even if you had a sample mean of 435 you would probably believe that the true mean was less than 500. What about 475? Or 483? There is some point where you would stop being so sure that the population mean is less than 500. That point separates the values of where you are sure or pretty sure that the mean is less than 500 from the area where you are not so sure. How do you find that point?

Well it depends on how much error you want to make. Of course you don’t want to make any errors, but unfortunately that is unavoidable in statistics. You need to figure out how much error you made with your sample. Take the sample mean, and find the probability of getting another sample mean less than it, assuming for the moment that the manufacturer is right. The idea behind this is that you want to know what is the chance that you could have come up with your sample mean even if the population mean really is 500 days.

You want to find \(P\left(\overline{x}<490 | H_{o} \text { is true }\right)=P(\overline{x}<490 | \mu=500)\)

To compute this probability, you need to know how the sample mean is distributed. Since the sample size is at least 30, then you know the sample mean is approximately normally distributed. Remember \(\mu_{\overline{x}}=\mu\) and \(\sigma_{\overline{x}}=\dfrac{\sigma}{\sqrt{n}}\)

A picture is always useful.

Before calculating the probability, it is useful to see how many standard deviations away from the mean the sample mean is. Using the formula for the z-score from chapter 6, you find

\(z=\dfrac{\overline{x}-\mu_{o}}{\sigma / \sqrt{n}}=\dfrac{490-500}{25 / \sqrt{30}}=-2.19\)

This sample mean is more than two standard deviations away from the mean. That seems pretty far, but you should look at the probability too.

On TI-83/84:

\(P(\overline{x}<490 | \mu=500)=\text { normalcdf }(-1 E 99,490,500,25 \div \sqrt{30}) \approx 0.0142\)

\(P(\overline{x}<490 \mu=500)=\text { pnorm }(490,500,25 / \operatorname{sqrt}(30)) \approx 0.0142\)

There is a 1.42% chance that you could find a sample mean less than 490 when the population mean is 500 days. This is really small, so the chances are that the assumption that the population mean is 500 days is wrong, and you can reject the manufacturer’s claim. But how do you quantify really small? Is 5% or 10% or 15% really small? How do you decide?

Before you answer that question, a couple more definitions are needed.

Definition \(\PageIndex{3}\)

Test Statistic : \(z=\dfrac{\overline{x}-\mu_{o}}{\sigma / \sqrt{n}}\) since it is calculated as part of the testing of the hypothesis.

Definition \(\PageIndex{4}\)

p – value : probability that the test statistic will take on more extreme values than the observed test statistic, given that the null hypothesis is true. It is the probability that was calculated above.

Now, how small is small enough? To answer that, you really want to know the types of errors you can make.

There are actually only two errors that can be made. The first error is if you say that \(H_{o}\) is false, when in fact it is true. This means you reject \(H_{o}\) when \(H_{o}\) was true. The second error is if you say that \(H_{o}\) is true, when in fact it is false. This means you fail to reject \(H_{o}\) when \(H_{o}\) is false. The following table organizes this for you:

Type of errors:

Definition \(\PageIndex{5}\)

Type I Error is rejecting \(H_{o}\) when \(H_{o}\) is true, and

Definition \(\PageIndex{6}\)

Type II Error is failing to reject \(H_{o}\) when \(H_{o}\) is false.

Since these are the errors, then one can define the probabilities attached to each error.

Definition \(\PageIndex{7}\)

\(\alpha\) = P(type I error) = P(rejecting \(H_{o} / H_{o}\) is true)

Definition \(\PageIndex{8}\)

\(\beta\) = P(type II error) = P(failing to reject \(H_{o} / H_{o}\) is false)

\(\alpha\) is also called the level of significance .

Another common concept that is used is Power = \(1-\beta \).

Now there is a relationship between \(\alpha\) and \(\beta\). They are not complements of each other. How are they related?

If \(\alpha\) increases that means the chances of making a type I error will increase. It is more likely that a type I error will occur. It makes sense that you are less likely to make type II errors, only because you will be rejecting \(H_{o}\) more often. You will be failing to reject \(H_{o}\) less, and therefore, the chance of making a type II error will decrease. Thus, as \(\alpha\) increases, \(\beta\) will decrease, and vice versa. That makes them seem like complements, but they aren’t complements. What gives? Consider one more factor – sample size.

Consider if you have a larger sample that is representative of the population, then it makes sense that you have more accuracy then with a smaller sample. Think of it this way, which would you trust more, a sample mean of 490 if you had a sample size of 35 or sample size of 350 (assuming a representative sample)? Of course the 350 because there are more data points and so more accuracy. If you are more accurate, then there is less chance that you will make any error. By increasing the sample size of a representative sample, you decrease both \(\alpha\) and \(\beta\).

Summary of all of this:

• For a certain sample size, n , if \(\alpha\) increases, \(\beta\) decreases.
• For a certain level of significance, \(\alpha\), if n increases, \(\beta\) decreases.

Now how do you find \(\alpha\) and \(\beta\)? Well \(\alpha\) is actually chosen. There are only three values that are usually picked for \(\alpha\): 0.01, 0.05, and 0.10. \(\beta\) is very difficult to find, so usually it isn’t found. If you want to make sure it is small you take as large of a sample as you can afford provided it is a representative sample. This is one use of the Power. You want \(\beta\) to be small and the Power of the test is large. The Power word sounds good.

Which pick of \(\alpha\) do you pick? Well that depends on what you are working on. Remember in this example you are the buyer who is trying to get out of a contract to buy these batteries. If you create a type I error, you said that the batteries are bad when they aren’t, most likely the manufacturer will sue you. You want to avoid this. You might pick \(\alpha\) to be 0.01. This way you have a small chance of making a type I error. Of course this means you have more of a chance of making a type II error. No big deal right? What if the batteries are used in pacemakers and you tell the person that their pacemaker’s batteries are good for 500 days when they actually last less, that might be bad. If you make a type II error, you say that the batteries do last 500 days when they last less, then you have the possibility of killing someone. You certainly do not want to do this. In this case you might want to pick \(\alpha\) as 0.10. If both errors are equally bad, then pick \(\alpha\) as 0.05.

The above discussion is why the choice of \(\alpha\) depends on what you are researching. As the researcher, you are the one that needs to decide what \(\alpha\) level to use based on your analysis of the consequences of making each error is.

If a type I error is really bad, then pick \(\alpha\) = 0.01.

If a type II error is really bad, then pick \(\alpha\) = 0.10

If neither error is bad, or both are equally bad, then pick \(\alpha\) = 0.05

The main thing is to always pick the \(\alpha\) before you collect the data and start the test.

The above discussion was long, but it is really important information. If you don’t know what the errors of the test are about, then there really is no point in making conclusions with the tests. Make sure you understand what the two errors are and what the probabilities are for them.

Now it is time to go back to the example and put this all together. This is the basic structure of testing a hypothesis, usually called a hypothesis test. Since this one has a test statistic involving z, it is also called a z-test. And since there is only one sample, it is usually called a one-sample z-test.

Example \(\PageIndex{2}\) battery example revisited

• State the random variable and the parameter in words.
• State the null and alternative hypothesis and the level of significance.
• A random sample of size n is taken.
• The population standard derivation is known.
• The sample size is at least 30 or the population of the random variable is normally distributed.
• Find the sample statistic, test statistic, and p-value.
• Interpretation

1. x = life of battery

\(\mu\) = mean life of a XJ35 battery

2. \(H_{o} : \mu=500\) days

\(H_{A} : \mu<500\) days

\(\alpha = 0.10\) (from above discussion about consequences)

3. Every hypothesis has some assumptions that be met to make sure that the results of the hypothesis are valid. The assumptions are different for each test. This test has the following assumptions.

• This occurred in this example, since it was stated that a random sample of 30 battery lives were taken.
• This is true, since it was given in the problem.
• The sample size was 30, so this condition is met.

4. The test statistic depends on how many samples there are, what parameter you are testing, and assumptions that need to be checked. In this case, there is one sample and you are testing the mean. The assumptions were checked above.

Sample statistic:

\(\overline{x} = 490\)

Test statistic:

Using TI-83/84:

\(P(\overline{x}<490 | \mu=500)=\text { normalcdf }(-1 \mathrm{E} 99,490,500,25 / \sqrt{30}) \approx 0.0142\)

\(P(\overline{x}<490 | \mu=500)=\operatorname{pnorm}(490,500,25 / \operatorname{sqrt}(30)) \approx 0.0142\)

5. Now what? Well, this p-value is 0.0142. This is a lot smaller than the amount of error you would accept in the problem -\(\alpha\) = 0.10. That means that finding a sample mean less than 490 days is unusual to happen if \(H_{o}\) is true. This should make you think that \(H_{o}\) is not true. You should reject \(H_{o}\).

In fact, in general:

Reject \(H_{o}\) if the p-value < \(\alpha\) and

Fail to reject \(H_{o}\) if the p-value \(\geq \alpha\).

6. Since you rejected \(H_{o}\), what does this mean in the real world? That is what goes in the interpretation. Since you rejected the claim by the manufacturer that the mean life of the batteries is 500 days, then you now can believe that your hypothesis was correct. In other words, there is enough evidence to show that the mean life of the battery is less than 500 days.

Now that you know that the batteries last less than 500 days, should you cancel the contract? Statistically, there is evidence that the batteries do not last as long as the manufacturer says they should. However, based on this sample there are only ten days less on average that the batteries last. There may not be practical significance in this case. Ten days do not seem like a large difference. In reality, if the batteries are used in pacemakers, then you would probably tell the patient to have the batteries replaced every year. You have a large buffer whether the batteries last 490 days or 500 days. It seems that it might not be worth it to break the contract over ten days. What if the 10 days was practically significant? Are there any other things you should consider? You might look at the business relationship with the manufacturer. You might also look at how much it would cost to find a new manufacturer. These are also questions to consider before making any changes. What this discussion should show you is that just because a hypothesis has statistical significance does not mean it has practical significance. The hypothesis test is just one part of a research process. There are other pieces that you need to consider.

That’s it. That is what a hypothesis test looks like. All hypothesis tests are done with the same six steps. Those general six steps are outlined below.

• State the random variable and the parameter in words. This is where you are defining what the unknowns are in this problem. x = random variable \(\mu\) = mean of random variable, if the parameter of interest is the mean. There are other parameters you can test, and you would use the appropriate symbol for that parameter.
• State the null and alternative hypotheses and the level of significance \(H_{o} : \mu=\mu_{o}\), where \(\mu_{o}\) is the known mean \(H_{A} : \mu<\mu_{o}\) \(H_{A} : \mu>\mu_{o}\), use the appropriate one for your problem \(H_{A} : \mu \neq \mu_{o}\) Also, state your \(\alpha\) level here.
• State and check the assumptions for a hypothesis test. Each hypothesis test has its own assumptions. They will be stated when the different hypothesis tests are discussed.
• Find the sample statistic, test statistic, and p-value. This depends on what parameter you are working with, how many samples, and the assumptions of the test. The p-value depends on your \(H_{A}\). If you are doing the \(H_{A}\) with the less than, then it is a left-tailed test, and you find the probability of being in that left tail. If you are doing the \(H_{A}\) with the greater than, then it is a right-tailed test, and you find the probability of being in the right tail. If you are doing the \(H_{A}\) with the not equal to, then you are doing a two-tail test, and you find the probability of being in both tails. Because of symmetry, you could find the probability in one tail and double this value to find the probability in both tails.
• Conclusion This is where you write reject \(H_{o}\) or fail to reject \(H_{o}\). The rule is: if the p-value < \(\alpha\), then reject \(H_{o}\). If the p-value \(\geq \alpha\), then fail to reject \(H_{o}\).
• Interpretation This is where you interpret in real world terms the conclusion to the test. The conclusion for a hypothesis test is that you either have enough evidence to show \(H_{A}\) is true, or you do not have enough evidence to show \(H_{A}\) is true.

Sorry, one more concept about the conclusion and interpretation. First, the conclusion is that you reject \(H_{o}\) or you fail to reject \(H_{o}\). Why was it said like this? It is because you never accept the null hypothesis. If you wanted to accept the null hypothesis, then why do the test in the first place? In the interpretation, you either have enough evidence to show \(H_{A}\) is true, or you do not have enough evidence to show \(H_{A}\) is true. You wouldn’t want to go to all this work and then find out you wanted to accept the claim. Why go through the trouble? You always want to show that the alternative hypothesis is true. Sometimes you can do that and sometimes you can’t. It doesn’t mean you proved the null hypothesis; it just means you can’t prove the alternative hypothesis. Here is an example to demonstrate this.

Example \(\PageIndex{3}\) conclusion in hypothesis tests

In the U.S. court system a jury trial could be set up as a hypothesis test. To really help you see how this works, let’s use OJ Simpson as an example. In the court system, a person is presumed innocent until he/she is proven guilty, and this is your null hypothesis. OJ Simpson was a football player in the 1970s. In 1994 his ex-wife and her friend were killed. OJ Simpson was accused of the crime, and in 1995 the case was tried. The prosecutors wanted to prove OJ was guilty of killing his wife and her friend, and that is the alternative hypothesis

\(H_{0}\): OJ is innocent of killing his wife and her friend

\(H_{A}\): OJ is guilty of killing his wife and her friend

In this case, a verdict of not guilty was given. That does not mean that he is innocent of this crime. It means there was not enough evidence to prove he was guilty. Many people believe that OJ was guilty of this crime, but the jury did not feel that the evidence presented was enough to show there was guilt. The verdict in a jury trial is always guilty or not guilty!

The same is true in a hypothesis test. There is either enough or not enough evidence to show that alternative hypothesis. It is not that you proved the null hypothesis true.

When identifying hypothesis, it is important to state your random variable and the appropriate parameter you want to make a decision about. If count something, then the random variable is the number of whatever you counted. The parameter is the proportion of what you counted. If the random variable is something you measured, then the parameter is the mean of what you measured. (Note: there are other parameters you can calculate, and some analysis of those will be presented in later chapters.)

Example \(\PageIndex{4}\) stating hypotheses

Identify the hypotheses necessary to test the following statements:

• The average salary of a teacher is more than $30,000.
• The proportion of students who like math is less than 10%.
• The average age of students in this class differs from 21.

a. x = salary of teacher

\(\mu\) = mean salary of teacher

The guess is that \(\mu>\$ 30,000\) and that is the alternative hypothesis.

The null hypothesis has the same parameter and number with an equal sign.

\(\begin{array}{l}{H_{0} : \mu=\$ 30,000} \\ {H_{A} : \mu>\$ 30,000}\end{array}\)

b. x = number od students who like math

p = proportion of students who like math

The guess is that p < 0.10 and that is the alternative hypothesis.

\(\begin{array}{l}{H_{0} : p=0.10} \\ {H_{A} : p<0.10}\end{array}\)

c. x = age of students in this class

\(\mu\) = mean age of students in this class

The guess is that \(\mu \neq 21\) and that is the alternative hypothesis.

\(\begin{array}{c}{H_{0} : \mu=21} \\ {H_{A} : \mu \neq 21}\end{array}\)

Example \(\PageIndex{5}\) Stating Type I and II Errors and Picking Level of Significance

• The plant-breeding department at a major university developed a new hybrid raspberry plant called YumYum Berry. Based on research data, the claim is made that from the time shoots are planted 90 days on average are required to obtain the first berry with a standard deviation of 9.2 days. A corporation that is interested in marketing the product tests 60 shoots by planting them and recording the number of days before each plant produces its first berry. The sample mean is 92.3 days. The corporation wants to know if the mean number of days is more than the 90 days claimed. State the type I and type II errors in terms of this problem, consequences of each error, and state which level of significance to use.
• A concern was raised in Australia that the percentage of deaths of Aboriginal prisoners was higher than the percent of deaths of non-indigenous prisoners, which is 0.27%. State the type I and type II errors in terms of this problem, consequences of each error, and state which level of significance to use.

a. x = time to first berry for YumYum Berry plant

\(\mu\) = mean time to first berry for YumYum Berry plant

\(\begin{array}{l}{H_{0} : \mu=90} \\ {H_{A} : \mu>90}\end{array}\)

Type I Error: If the corporation does a type I error, then they will say that the plants take longer to produce than 90 days when they don’t. They probably will not want to market the plants if they think they will take longer. They will not market them even though in reality the plants do produce in 90 days. They may have loss of future earnings, but that is all.

Type II error: The corporation do not say that the plants take longer then 90 days to produce when they do take longer. Most likely they will market the plants. The plants will take longer, and so customers might get upset and then the company would get a bad reputation. This would be really bad for the company.

Level of significance: It appears that the corporation would not want to make a type II error. Pick a 10% level of significance, \(\alpha = 0.10\).

b. x = number of Aboriginal prisoners who have died

p = proportion of Aboriginal prisoners who have died

\(\begin{array}{l}{H_{o} : p=0.27 \%} \\ {H_{A} : p>0.27 \%}\end{array}\)

Type I error: Rejecting that the proportion of Aboriginal prisoners who died was 0.27%, when in fact it was 0.27%. This would mean you would say there is a problem when there isn’t one. You could anger the Aboriginal community, and spend time and energy researching something that isn’t a problem.

Type II error: Failing to reject that the proportion of Aboriginal prisoners who died was 0.27%, when in fact it is higher than 0.27%. This would mean that you wouldn’t think there was a problem with Aboriginal prisoners dying when there really is a problem. You risk causing deaths when there could be a way to avoid them.

Level of significance: It appears that both errors may be issues in this case. You wouldn’t want to anger the Aboriginal community when there isn’t an issue, and you wouldn’t want people to die when there may be a way to stop it. It may be best to pick a 5% level of significance, \(\alpha = 0.05\).

Hypothesis testing is really easy if you follow the same recipe every time. The only differences in the various problems are the assumptions of the test and the test statistic you calculate so you can find the p-value. Do the same steps, in the same order, with the same words, every time and these problems become very easy.

Exercise \(\PageIndex{1}\)

For the problems in this section, a question is being asked. This is to help you understand what the hypotheses are. You are not to run any hypothesis tests and come up with any conclusions in this section.

• Eyeglassomatic manufactures eyeglasses for different retailers. They test to see how many defective lenses they made in a given time period and found that 11% of all lenses had defects of some type. Looking at the type of defects, they found in a three-month time period that out of 34,641 defective lenses, 5865 were due to scratches. Are there more defects from scratches than from all other causes? State the random variable, population parameter, and hypotheses.
• According to the February 2008 Federal Trade Commission report on consumer fraud and identity theft, 23% of all complaints in 2007 were for identity theft. In that year, Alaska had 321 complaints of identity theft out of 1,432 consumer complaints ("Consumer fraud and," 2008). Does this data provide enough evidence to show that Alaska had a lower proportion of identity theft than 23%? State the random variable, population parameter, and hypotheses.
• The Kyoto Protocol was signed in 1997, and required countries to start reducing their carbon emissions. The protocol became enforceable in February 2005. In 2004, the mean CO2 emission was 4.87 metric tons per capita. Is there enough evidence to show that the mean CO2 emission is lower in 2010 than in 2004? State the random variable, population parameter, and hypotheses.
• The FDA regulates that fish that is consumed is allowed to contain 1.0 mg/kg of mercury. In Florida, bass fish were collected in 53 different lakes to measure the amount of mercury in the fish. The data for the average amount of mercury in each lake is in Example \(\PageIndex{5}\) ("Multi-disciplinary niser activity," 2013). Do the data provide enough evidence to show that the fish in Florida lakes has more mercury than the allowable amount? State the random variable, population parameter, and hypotheses.
• Eyeglassomatic manufactures eyeglasses for different retailers. They test to see how many defective lenses they made in a given time period and found that 11% of all lenses had defects of some type. Looking at the type of defects, they found in a three-month time period that out of 34,641 defective lenses, 5865 were due to scratches. Are there more defects from scratches than from all other causes? State the type I and type II errors in this case, consequences of each error type for this situation from the perspective of the manufacturer, and the appropriate alpha level to use. State why you picked this alpha level.
• According to the February 2008 Federal Trade Commission report on consumer fraud and identity theft, 23% of all complaints in 2007 were for identity theft. In that year, Alaska had 321 complaints of identity theft out of 1,432 consumer complaints ("Consumer fraud and," 2008). Does this data provide enough evidence to show that Alaska had a lower proportion of identity theft than 23%? State the type I and type II errors in this case, consequences of each error type for this situation from the perspective of the state of Arizona, and the appropriate alpha level to use. State why you picked this alpha level.
• The Kyoto Protocol was signed in 1997, and required countries to start reducing their carbon emissions. The protocol became enforceable in February 2005. In 2004, the mean CO2 emission was 4.87 metric tons per capita. Is there enough evidence to show that the mean CO2 emission is lower in 2010 than in 2004? State the type I and type II errors in this case, consequences of each error type for this situation from the perspective of the agency overseeing the protocol, and the appropriate alpha level to use. State why you picked this alpha level.
• The FDA regulates that fish that is consumed is allowed to contain 1.0 mg/kg of mercury. In Florida, bass fish were collected in 53 different lakes to measure the amount of mercury in the fish. The data for the average amount of mercury in each lake is in Example \(\PageIndex{5}\) ("Multi-disciplinary niser activity," 2013). Do the data provide enough evidence to show that the fish in Florida lakes has more mercury than the allowable amount? State the type I and type II errors in this case, consequences of each error type for this situation from the perspective of the FDA, and the appropriate alpha level to use. State why you picked this alpha level.

1. \(H_{o} : p=0.11, H_{A} : p>0.11\)

3. \(H_{o} : \mu=4.87 \text { metric tons per capita, } H_{A} : \mu<4.87 \text { metric tons per capita }\)

5. See solutions

7. See solutions

What Is a Hypothesis? (Science)

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A hypothesis (plural hypotheses) is a proposed explanation for an observation. The definition depends on the subject.

In science, a hypothesis is part of the scientific method. It is a prediction or explanation that is tested by an experiment. Observations and experiments may disprove a scientific hypothesis, but can never entirely prove one.

In the study of logic, a hypothesis is an if-then proposition, typically written in the form, "If X , then Y ."

In common usage, a hypothesis is simply a proposed explanation or prediction, which may or may not be tested.

Writing a Hypothesis

Most scientific hypotheses are proposed in the if-then format because it's easy to design an experiment to see whether or not a cause and effect relationship exists between the independent variable and the dependent variable . The hypothesis is written as a prediction of the outcome of the experiment.

• Null Hypothesis and Alternative Hypothesis

Statistically, it's easier to show there is no relationship between two variables than to support their connection. So, scientists often propose the null hypothesis . The null hypothesis assumes changing the independent variable will have no effect on the dependent variable.

In contrast, the alternative hypothesis suggests changing the independent variable will have an effect on the dependent variable. Designing an experiment to test this hypothesis can be trickier because there are many ways to state an alternative hypothesis.

For example, consider a possible relationship between getting a good night's sleep and getting good grades. The null hypothesis might be stated: "The number of hours of sleep students get is unrelated to their grades" or "There is no correlation between hours of sleep and grades."

An experiment to test this hypothesis might involve collecting data, recording average hours of sleep for each student and grades. If a student who gets eight hours of sleep generally does better than students who get four hours of sleep or 10 hours of sleep, the hypothesis might be rejected.

But the alternative hypothesis is harder to propose and test. The most general statement would be: "The amount of sleep students get affects their grades." The hypothesis might also be stated as "If you get more sleep, your grades will improve" or "Students who get nine hours of sleep have better grades than those who get more or less sleep."

In an experiment, you can collect the same data, but the statistical analysis is less likely to give you a high confidence limit.

Usually, a scientist starts out with the null hypothesis. From there, it may be possible to propose and test an alternative hypothesis, to narrow down the relationship between the variables.

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In today’s data-driven world , decisions are based on data all the time. Hypothesis plays a crucial role in that process, whether it may be making business decisions, in the health sector, academia, or in quality improvement. Without hypothesis & hypothesis tests, you risk drawing the wrong conclusions and making bad decisions. In this tutorial, you will look at Hypothesis Testing in Statistics.

What Is Hypothesis Testing in Statistics?

Hypothesis Testing is a type of statistical analysis in which you put your assumptions about a population parameter to the test. It is used to estimate the relationship between 2 statistical variables.

Let's discuss few examples of statistical hypothesis from real-life - 

• A teacher assumes that 60% of his college's students come from lower-middle-class families.
• A doctor believes that 3D (Diet, Dose, and Discipline) is 90% effective for diabetic patients.

Now that you know about hypothesis testing, look at the two types of hypothesis testing in statistics.

Hypothesis Testing Formula

Z = ( x̅ – μ0 ) / (σ /√n)

• Here, x̅ is the sample mean,
• μ0 is the population mean,
• σ is the standard deviation,
• n is the sample size.

How Hypothesis Testing Works?

An analyst performs hypothesis testing on a statistical sample to present evidence of the plausibility of the null hypothesis. Measurements and analyses are conducted on a random sample of the population to test a theory. Analysts use a random population sample to test two hypotheses: the null and alternative hypotheses.

The null hypothesis is typically an equality hypothesis between population parameters; for example, a null hypothesis may claim that the population means return equals zero. The alternate hypothesis is essentially the inverse of the null hypothesis (e.g., the population means the return is not equal to zero). As a result, they are mutually exclusive, and only one can be correct. One of the two possibilities, however, will always be correct.

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Null Hypothesis and Alternate Hypothesis

The Null Hypothesis is the assumption that the event will not occur. A null hypothesis has no bearing on the study's outcome unless it is rejected.

H0 is the symbol for it, and it is pronounced H-naught.

The Alternate Hypothesis is the logical opposite of the null hypothesis. The acceptance of the alternative hypothesis follows the rejection of the null hypothesis. H1 is the symbol for it.

Let's understand this with an example.

A sanitizer manufacturer claims that its product kills 95 percent of germs on average. 

To put this company's claim to the test, create a null and alternate hypothesis.

H0 (Null Hypothesis): Average = 95%.

Alternative Hypothesis (H1): The average is less than 95%.

Another straightforward example to understand this concept is determining whether or not a coin is fair and balanced. The null hypothesis states that the probability of a show of heads is equal to the likelihood of a show of tails. In contrast, the alternate theory states that the probability of a show of heads and tails would be very different.

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Hypothesis Testing Calculation With Examples

Let's consider a hypothesis test for the average height of women in the United States. Suppose our null hypothesis is that the average height is 5'4". We gather a sample of 100 women and determine that their average height is 5'5". The standard deviation of population is 2.

To calculate the z-score, we would use the following formula:

z = ( x̅ – μ0 ) / (σ /√n)

z = (5'5" - 5'4") / (2" / √100)

z = 0.5 / (0.045)

 We will reject the null hypothesis as the z-score of 11.11 is very large and conclude that there is evidence to suggest that the average height of women in the US is greater than 5'4".

Steps of Hypothesis Testing

Step 1: specify your null and alternate hypotheses.

It is critical to rephrase your original research hypothesis (the prediction that you wish to study) as a null (Ho) and alternative (Ha) hypothesis so that you can test it quantitatively. Your first hypothesis, which predicts a link between variables, is generally your alternate hypothesis. The null hypothesis predicts no link between the variables of interest.

Step 2: Gather Data

For a statistical test to be legitimate, sampling and data collection must be done in a way that is meant to test your hypothesis. You cannot draw statistical conclusions about the population you are interested in if your data is not representative.

Step 3: Conduct a Statistical Test

Other statistical tests are available, but they all compare within-group variance (how to spread out the data inside a category) against between-group variance (how different the categories are from one another). If the between-group variation is big enough that there is little or no overlap between groups, your statistical test will display a low p-value to represent this. This suggests that the disparities between these groups are unlikely to have occurred by accident. Alternatively, if there is a large within-group variance and a low between-group variance, your statistical test will show a high p-value. Any difference you find across groups is most likely attributable to chance. The variety of variables and the level of measurement of your obtained data will influence your statistical test selection.

Step 4: Determine Rejection Of Your Null Hypothesis

Your statistical test results must determine whether your null hypothesis should be rejected or not. In most circumstances, you will base your judgment on the p-value provided by the statistical test. In most circumstances, your preset level of significance for rejecting the null hypothesis will be 0.05 - that is, when there is less than a 5% likelihood that these data would be seen if the null hypothesis were true. In other circumstances, researchers use a lower level of significance, such as 0.01 (1%). This reduces the possibility of wrongly rejecting the null hypothesis.

Step 5: Present Your Results 

The findings of hypothesis testing will be discussed in the results and discussion portions of your research paper, dissertation, or thesis. You should include a concise overview of the data and a summary of the findings of your statistical test in the results section. You can talk about whether your results confirmed your initial hypothesis or not in the conversation. Rejecting or failing to reject the null hypothesis is a formal term used in hypothesis testing. This is likely a must for your statistics assignments.

Types of Hypothesis Testing

To determine whether a discovery or relationship is statistically significant, hypothesis testing uses a z-test. It usually checks to see if two means are the same (the null hypothesis). Only when the population standard deviation is known and the sample size is 30 data points or more, can a z-test be applied.

A statistical test called a t-test is employed to compare the means of two groups. To determine whether two groups differ or if a procedure or treatment affects the population of interest, it is frequently used in hypothesis testing.

Chi-Square 

You utilize a Chi-square test for hypothesis testing concerning whether your data is as predicted. To determine if the expected and observed results are well-fitted, the Chi-square test analyzes the differences between categorical variables from a random sample. The test's fundamental premise is that the observed values in your data should be compared to the predicted values that would be present if the null hypothesis were true.

Hypothesis Testing and Confidence Intervals

Both confidence intervals and hypothesis tests are inferential techniques that depend on approximating the sample distribution. Data from a sample is used to estimate a population parameter using confidence intervals. Data from a sample is used in hypothesis testing to examine a given hypothesis. We must have a postulated parameter to conduct hypothesis testing.

Bootstrap distributions and randomization distributions are created using comparable simulation techniques. The observed sample statistic is the focal point of a bootstrap distribution, whereas the null hypothesis value is the focal point of a randomization distribution.

A variety of feasible population parameter estimates are included in confidence ranges. In this lesson, we created just two-tailed confidence intervals. There is a direct connection between these two-tail confidence intervals and these two-tail hypothesis tests. The results of a two-tailed hypothesis test and two-tailed confidence intervals typically provide the same results. In other words, a hypothesis test at the 0.05 level will virtually always fail to reject the null hypothesis if the 95% confidence interval contains the predicted value. A hypothesis test at the 0.05 level will nearly certainly reject the null hypothesis if the 95% confidence interval does not include the hypothesized parameter.

Simple and Composite Hypothesis Testing

Depending on the population distribution, you can classify the statistical hypothesis into two types.

Simple Hypothesis: A simple hypothesis specifies an exact value for the parameter.

Composite Hypothesis: A composite hypothesis specifies a range of values.

A company is claiming that their average sales for this quarter are 1000 units. This is an example of a simple hypothesis.

Suppose the company claims that the sales are in the range of 900 to 1000 units. Then this is a case of a composite hypothesis.

One-Tailed and Two-Tailed Hypothesis Testing

The One-Tailed test, also called a directional test, considers a critical region of data that would result in the null hypothesis being rejected if the test sample falls into it, inevitably meaning the acceptance of the alternate hypothesis.

In a one-tailed test, the critical distribution area is one-sided, meaning the test sample is either greater or lesser than a specific value.

In two tails, the test sample is checked to be greater or less than a range of values in a Two-Tailed test, implying that the critical distribution area is two-sided.

If the sample falls within this range, the alternate hypothesis will be accepted, and the null hypothesis will be rejected.

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Right Tailed Hypothesis Testing

If the larger than (>) sign appears in your hypothesis statement, you are using a right-tailed test, also known as an upper test. Or, to put it another way, the disparity is to the right. For instance, you can contrast the battery life before and after a change in production. Your hypothesis statements can be the following if you want to know if the battery life is longer than the original (let's say 90 hours):

• The null hypothesis is (H0 <= 90) or less change.
• A possibility is that battery life has risen (H1) > 90.

The crucial point in this situation is that the alternate hypothesis (H1), not the null hypothesis, decides whether you get a right-tailed test.

Left Tailed Hypothesis Testing

Alternative hypotheses that assert the true value of a parameter is lower than the null hypothesis are tested with a left-tailed test; they are indicated by the asterisk "<".

Suppose H0: mean = 50 and H1: mean not equal to 50

According to the H1, the mean can be greater than or less than 50. This is an example of a Two-tailed test.

In a similar manner, if H0: mean >=50, then H1: mean <50

Here the mean is less than 50. It is called a One-tailed test.

Type 1 and Type 2 Error

A hypothesis test can result in two types of errors.

Type 1 Error: A Type-I error occurs when sample results reject the null hypothesis despite being true.

Type 2 Error: A Type-II error occurs when the null hypothesis is not rejected when it is false, unlike a Type-I error.

Suppose a teacher evaluates the examination paper to decide whether a student passes or fails.

H0: Student has passed

H1: Student has failed

Type I error will be the teacher failing the student [rejects H0] although the student scored the passing marks [H0 was true]. 

Type II error will be the case where the teacher passes the student [do not reject H0] although the student did not score the passing marks [H1 is true].

Level of Significance

The alpha value is a criterion for determining whether a test statistic is statistically significant. In a statistical test, Alpha represents an acceptable probability of a Type I error. Because alpha is a probability, it can be anywhere between 0 and 1. In practice, the most commonly used alpha values are 0.01, 0.05, and 0.1, which represent a 1%, 5%, and 10% chance of a Type I error, respectively (i.e. rejecting the null hypothesis when it is in fact correct).

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A p-value is a metric that expresses the likelihood that an observed difference could have occurred by chance. As the p-value decreases the statistical significance of the observed difference increases. If the p-value is too low, you reject the null hypothesis.

Here you have taken an example in which you are trying to test whether the new advertising campaign has increased the product's sales. The p-value is the likelihood that the null hypothesis, which states that there is no change in the sales due to the new advertising campaign, is true. If the p-value is .30, then there is a 30% chance that there is no increase or decrease in the product's sales.  If the p-value is 0.03, then there is a 3% probability that there is no increase or decrease in the sales value due to the new advertising campaign. As you can see, the lower the p-value, the chances of the alternate hypothesis being true increases, which means that the new advertising campaign causes an increase or decrease in sales.

Why is Hypothesis Testing Important in Research Methodology?

Hypothesis testing is crucial in research methodology for several reasons:

• Provides evidence-based conclusions: It allows researchers to make objective conclusions based on empirical data, providing evidence to support or refute their research hypotheses.
• Supports decision-making: It helps make informed decisions, such as accepting or rejecting a new treatment, implementing policy changes, or adopting new practices.
• Adds rigor and validity: It adds scientific rigor to research using statistical methods to analyze data, ensuring that conclusions are based on sound statistical evidence.
• Contributes to the advancement of knowledge: By testing hypotheses, researchers contribute to the growth of knowledge in their respective fields by confirming existing theories or discovering new patterns and relationships.

Limitations of Hypothesis Testing

Hypothesis testing has some limitations that researchers should be aware of:

• It cannot prove or establish the truth: Hypothesis testing provides evidence to support or reject a hypothesis, but it cannot confirm the absolute truth of the research question.
• Results are sample-specific: Hypothesis testing is based on analyzing a sample from a population, and the conclusions drawn are specific to that particular sample.
• Possible errors: During hypothesis testing, there is a chance of committing type I error (rejecting a true null hypothesis) or type II error (failing to reject a false null hypothesis).
• Assumptions and requirements: Different tests have specific assumptions and requirements that must be met to accurately interpret results.

After reading this tutorial, you would have a much better understanding of hypothesis testing, one of the most important concepts in the field of Data Science . The majority of hypotheses are based on speculation about observed behavior, natural phenomena, or established theories.

If you are interested in statistics of data science and skills needed for such a career, you ought to explore Simplilearn’s Post Graduate Program in Data Science.

If you have any questions regarding this ‘Hypothesis Testing In Statistics’ tutorial, do share them in the comment section. Our subject matter expert will respond to your queries. Happy learning!

1. What is hypothesis testing in statistics with example?

Hypothesis testing is a statistical method used to determine if there is enough evidence in a sample data to draw conclusions about a population. It involves formulating two competing hypotheses, the null hypothesis (H0) and the alternative hypothesis (Ha), and then collecting data to assess the evidence. An example: testing if a new drug improves patient recovery (Ha) compared to the standard treatment (H0) based on collected patient data.

2. What is hypothesis testing and its types?

Hypothesis testing is a statistical method used to make inferences about a population based on sample data. It involves formulating two hypotheses: the null hypothesis (H0), which represents the default assumption, and the alternative hypothesis (Ha), which contradicts H0. The goal is to assess the evidence and determine whether there is enough statistical significance to reject the null hypothesis in favor of the alternative hypothesis.

Types of hypothesis testing:

• One-sample test: Used to compare a sample to a known value or a hypothesized value.
• Two-sample test: Compares two independent samples to assess if there is a significant difference between their means or distributions.
• Paired-sample test: Compares two related samples, such as pre-test and post-test data, to evaluate changes within the same subjects over time or under different conditions.
• Chi-square test: Used to analyze categorical data and determine if there is a significant association between variables.
• ANOVA (Analysis of Variance): Compares means across multiple groups to check if there is a significant difference between them.

3. What are the steps of hypothesis testing?

The steps of hypothesis testing are as follows:

• Formulate the hypotheses: State the null hypothesis (H0) and the alternative hypothesis (Ha) based on the research question.
• Set the significance level: Determine the acceptable level of error (alpha) for making a decision.
• Collect and analyze data: Gather and process the sample data.
• Compute test statistic: Calculate the appropriate statistical test to assess the evidence.
• Make a decision: Compare the test statistic with critical values or p-values and determine whether to reject H0 in favor of Ha or not.
• Draw conclusions: Interpret the results and communicate the findings in the context of the research question.

4. What are the 2 types of hypothesis testing?

• One-tailed (or one-sided) test: Tests for the significance of an effect in only one direction, either positive or negative.
• Two-tailed (or two-sided) test: Tests for the significance of an effect in both directions, allowing for the possibility of a positive or negative effect.

The choice between one-tailed and two-tailed tests depends on the specific research question and the directionality of the expected effect.

5. What are the 3 major types of hypothesis?

The three major types of hypotheses are:

• Null Hypothesis (H0): Represents the default assumption, stating that there is no significant effect or relationship in the data.
• Alternative Hypothesis (Ha): Contradicts the null hypothesis and proposes a specific effect or relationship that researchers want to investigate.
• Nondirectional Hypothesis: An alternative hypothesis that doesn't specify the direction of the effect, leaving it open for both positive and negative possibilities.

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How to Test a Hypothesis

Last Updated: July 4, 2023 Fact Checked

This article was co-authored by Meredith Juncker, PhD . Meredith Juncker is a PhD candidate in Biochemistry and Molecular Biology at Louisiana State University Health Sciences Center. Her studies are focused on proteins and neurodegenerative diseases. There are 8 references cited in this article, which can be found at the bottom of the page. This article has been fact-checked, ensuring the accuracy of any cited facts and confirming the authority of its sources. This article has been viewed 40,473 times.

Testing a hypothesis is an important part of the scientific method. It allows you to evaluate the validity of an educated guess. In a typical process, you’ll form a hypothesis based on evidence that you’ve gathered, and then test that hypothesis through experiments. As you gather more and more data, you’ll be able to see if your original hypothesis was correct. If there were flaws in your first guess, you can revise your hypothesis to better match what you’ve learned from your data.

Asking a Question and Researching

• For example, a question could be something like, “Which brand of stain remover will remove stains from fabrics most effectively?” [2] X Research source

• For the stain-remover experiment, you could dirty 4 types of fabric (e.g. cotton, linen, wool, polyester) each with 4 different types of stains (e.g. red wine, grass, mud and dirt, grease), and then test the top four or five brands of stain remover (e.g. Mr. Clean, Tide, Shout, Clorox) to see which removes the largest number of stains.

• In the case of the stain-remover experiment, you’d need to purchase a bottle of each of the major stain-remover brands and dirty a variety of fabrics with a variety of stains.
• Then, test out each type of detergent on each of the stained fabrics. (If you live at your parents’ house, you’ll need to get permission to use the laundry room for most of a day.)

Making and Challenging Your Hypothesis

• For instance, if you’ve run some loads of laundry (maybe testing which brand of stain remover works best on removing different stains from linen), you can use your results to take a stab at a hypothesis.
• A good working hypothesis would look like: “If fabrics are stained with common household items, Tide stain remover will remove the stains most effectively.”

• In our example, since you only tested 1 type of fabric (linen), you’ll need to repeat the laundry test with the other 3 fabrics (cotton, wool, polyester) and note which stain remover most effectively eliminates the stains.

• While it can be tempting to only accept data that supports your hypothesis, it’s not scientific or ethical.
• You must accept all the data and watch for whatever patterns appear, even if it proves your hypothesis to be likely false.
• Note that significant results don’t mean your hypothesis is proven, but rather that based on the data you collected, the differences you observed are likely not due to chance.

Revising Your Hypothesis

• For example, if you began the experiment thinking that Tide would have the most effective stain remover, but you’ve noticed that Tide does a poor job of removing stains from red wine and mud, you likely need to change your working assumptions.

• So, if Tide turns out to be ineffective at removing certain types of stains, your early working hypothesis will have been incorrect.

• A final, tested hypothesis would look like: “Shout is the most effective stain remover for removing a variety of household stains from a variety of common fabrics.”

Expert Q&A

• Deductive (or “top-down”) reasoning won’t help you much in testing out a scientific hypothesis. Your hypothesis needs to be grounded in the tests you’ve run and the data that you’ve gathered. Thanks Helpful 0 Not Helpful 0
• Depending on the type of hypothesis you’re testing, you may also need a control group. For example, if you’re testing how effective a drug is, you need to have a group on a placebo. Thanks Helpful 0 Not Helpful 0
• Remember that a null hypothesis (when the control and tested variable are the same) is different from an alternative hypothesis (when the control and tested variable are different). Thanks Helpful 0 Not Helpful 0

You Might Also Like

• ↑ https://grammar.yourdictionary.com/for-students-and-parents/how-create-hypothesis.html
• ↑ https://www.education.com/science-fair/article/best-way-stains-out-of-clothes/
• ↑ https://www.grammarly.com/blog/how-to-write-a-hypothesis/
• ↑ https://www.sciencebuddies.org/science-fair-projects/science-fair/steps-of-the-scientific-method
• ↑ https://online.stat.psu.edu/stat502_fa21/lesson/1/1.1
• ↑ https://pressbooks.pub/scientificinquiryinsocialwork/chapter/6-3-inductive-and-deductive-reasoning/
• ↑ https://online.stat.psu.edu/stat100/lesson/10/10.1
• ↑ https://www.khanacademy.org/science/biology/intro-to-biology/science-of-biology/a/the-science-of-biology

About this article

If you need to test a hypothesis, first come up with a question you’d like to answer, then develop an experiment to answer your question. As you set up your experiment, create a statement about what you think is happening. This is your hypothesis. As you perform the test, compare your data to your hypothesis. By the end of the experiment, you should be able to conclude whether your hypothesis was true or not. Read on to learn more from our Science co-author about how to set up an experiment! Did this summary help you? Yes No

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Hypothesis testing involves formulating assumptions about population parameters based on sample statistics and rigorously evaluating these assumptions against empirical evidence. This article sheds light on the significance of hypothesis testing and the critical steps involved in the process.

What is Hypothesis Testing?

Hypothesis testing is a statistical method that is used to make a statistical decision using experimental data. Hypothesis testing is basically an assumption that we make about a population parameter. It evaluates two mutually exclusive statements about a population to determine which statement is best supported by the sample data. 

Example: You say an average height in the class is 30 or a boy is taller than a girl. All of these is an assumption that we are assuming, and we need some statistical way to prove these. We need some mathematical conclusion whatever we are assuming is true.

Defining Hypotheses

Key Terms of Hypothesis Testing

• P-value: The P value , or calculated probability, is the probability of finding the observed/extreme results when the null hypothesis(H0) of a study-given problem is true. If your P-value is less than the chosen significance level then you reject the null hypothesis i.e. accept that your sample claims to support the alternative hypothesis.
• Test Statistic: The test statistic is a numerical value calculated from sample data during a hypothesis test, used to determine whether to reject the null hypothesis. It is compared to a critical value or p-value to make decisions about the statistical significance of the observed results.
• Critical value : The critical value in statistics is a threshold or cutoff point used to determine whether to reject the null hypothesis in a hypothesis test.
• Degrees of freedom: Degrees of freedom are associated with the variability or freedom one has in estimating a parameter. The degrees of freedom are related to the sample size and determine the shape.

Why do we use Hypothesis Testing?

Hypothesis testing is an important procedure in statistics. Hypothesis testing evaluates two mutually exclusive population statements to determine which statement is most supported by sample data. When we say that the findings are statistically significant, thanks to hypothesis testing. 

One-Tailed and Two-Tailed Test

One tailed test focuses on one direction, either greater than or less than a specified value. We use a one-tailed test when there is a clear directional expectation based on prior knowledge or theory. The critical region is located on only one side of the distribution curve. If the sample falls into this critical region, the null hypothesis is rejected in favor of the alternative hypothesis.

One-Tailed Test

There are two types of one-tailed test:

Two-Tailed Test

A two-tailed test considers both directions, greater than and less than a specified value.We use a two-tailed test when there is no specific directional expectation, and want to detect any significant difference.

What are Type 1 and Type 2 errors in Hypothesis Testing?

In hypothesis testing, Type I and Type II errors are two possible errors that researchers can make when drawing conclusions about a population based on a sample of data. These errors are associated with the decisions made regarding the null hypothesis and the alternative hypothesis.

How does Hypothesis Testing work?

Step 1: define null and alternative hypothesis.

We first identify the problem about which we want to make an assumption keeping in mind that our assumption should be contradictory to one another, assuming Normally distributed data.

Step 2 – Choose significance level

Step 3 – Collect and Analyze data.

Gather relevant data through observation or experimentation. Analyze the data using appropriate statistical methods to obtain a test statistic.

Step 4-Calculate Test Statistic

The data for the tests are evaluated in this step we look for various scores based on the characteristics of data. The choice of the test statistic depends on the type of hypothesis test being conducted.

There are various hypothesis tests, each appropriate for various goal to calculate our test. This could be a Z-test , Chi-square , T-test , and so on.

• Z-test : If population means and standard deviations are known. Z-statistic is commonly used.
• t-test : If population standard deviations are unknown. and sample size is small than t-test statistic is more appropriate.
• Chi-square test : Chi-square test is used for categorical data or for testing independence in contingency tables
• F-test : F-test is often used in analysis of variance (ANOVA) to compare variances or test the equality of means across multiple groups.

We have a smaller dataset, So, T-test is more appropriate to test our hypothesis.

T-statistic is a measure of the difference between the means of two groups relative to the variability within each group. It is calculated as the difference between the sample means divided by the standard error of the difference. It is also known as the t-value or t-score.

Step 5 – Comparing Test Statistic:

In this stage, we decide where we should accept the null hypothesis or reject the null hypothesis. There are two ways to decide where we should accept or reject the null hypothesis.

Method A: Using Crtical values

Comparing the test statistic and tabulated critical value we have,

• If Test Statistic>Critical Value: Reject the null hypothesis.
• If Test Statistic≤Critical Value: Fail to reject the null hypothesis.

Note: Critical values are predetermined threshold values that are used to make a decision in hypothesis testing. To determine critical values for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Method B: Using P-values

We can also come to an conclusion using the p-value,

Note : The p-value is the probability of obtaining a test statistic as extreme as, or more extreme than, the one observed in the sample, assuming the null hypothesis is true. To determine p-value for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Step 7- Interpret the Results

At last, we can conclude our experiment using method A or B.

Calculating test statistic

To validate our hypothesis about a population parameter we use statistical functions . We use the z-score, p-value, and level of significance(alpha) to make evidence for our hypothesis for normally distributed data .

1. Z-statistics:

When population means and standard deviations are known.

• μ represents the population mean, 
• σ is the standard deviation
• and n is the size of the sample.

2. T-Statistics

T test is used when n<30,

t-statistic calculation is given by:

• t = t-score,
• x̄ = sample mean
• μ = population mean,
• s = standard deviation of the sample,
• n = sample size

3. Chi-Square Test

Chi-Square Test for Independence categorical Data (Non-normally distributed) using:

• i,j are the rows and columns index respectively.

Real life Hypothesis Testing example

Let’s examine hypothesis testing using two real life situations,

Case A: D oes a New Drug Affect Blood Pressure?

Imagine a pharmaceutical company has developed a new drug that they believe can effectively lower blood pressure in patients with hypertension. Before bringing the drug to market, they need to conduct a study to assess its impact on blood pressure.

• Before Treatment: 120, 122, 118, 130, 125, 128, 115, 121, 123, 119
• After Treatment: 115, 120, 112, 128, 122, 125, 110, 117, 119, 114

Step 1 : Define the Hypothesis

• Null Hypothesis : (H 0 )The new drug has no effect on blood pressure.
• Alternate Hypothesis : (H 1 )The new drug has an effect on blood pressure.

Step 2: Define the Significance level

Let’s consider the Significance level at 0.05, indicating rejection of the null hypothesis.

If the evidence suggests less than a 5% chance of observing the results due to random variation.

Step 3 : Compute the test statistic

Using paired T-test analyze the data to obtain a test statistic and a p-value.

The test statistic (e.g., T-statistic) is calculated based on the differences between blood pressure measurements before and after treatment.

t = m/(s/√n)

• m  = mean of the difference i.e X after, X before
• s  = standard deviation of the difference (d) i.e d i ​= X after, i ​− X before,
• n  = sample size,

then, m= -3.9, s= 1.8 and n= 10

we, calculate the , T-statistic = -9 based on the formula for paired t test

Step 4: Find the p-value

The calculated t-statistic is -9 and degrees of freedom df = 9, you can find the p-value using statistical software or a t-distribution table.

thus, p-value = 8.538051223166285e-06

Step 5: Result

• If the p-value is less than or equal to 0.05, the researchers reject the null hypothesis.
• If the p-value is greater than 0.05, they fail to reject the null hypothesis.

Conclusion: Since the p-value (8.538051223166285e-06) is less than the significance level (0.05), the researchers reject the null hypothesis. There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different.

Python Implementation of Hypothesis Testing

Let’s create hypothesis testing with python, where we are testing whether a new drug affects blood pressure. For this example, we will use a paired T-test. We’ll use the scipy.stats library for the T-test.

Scipy is a mathematical library in Python that is mostly used for mathematical equations and computations.

We will implement our first real life problem via python,

In the above example, given the T-statistic of approximately -9 and an extremely small p-value, the results indicate a strong case to reject the null hypothesis at a significance level of 0.05. 

• The results suggest that the new drug, treatment, or intervention has a significant effect on lowering blood pressure.
• The negative T-statistic indicates that the mean blood pressure after treatment is significantly lower than the assumed population mean before treatment.

Case B : Cholesterol level in a population

Data: A sample of 25 individuals is taken, and their cholesterol levels are measured.

Cholesterol Levels (mg/dL): 205, 198, 210, 190, 215, 205, 200, 192, 198, 205, 198, 202, 208, 200, 205, 198, 205, 210, 192, 205, 198, 205, 210, 192, 205.

Populations Mean = 200

Population Standard Deviation (σ): 5 mg/dL(given for this problem)

Step 1: Define the Hypothesis

• Null Hypothesis (H 0 ): The average cholesterol level in a population is 200 mg/dL.
• Alternate Hypothesis (H 1 ): The average cholesterol level in a population is different from 200 mg/dL.

As the direction of deviation is not given , we assume a two-tailed test, and based on a normal distribution table, the critical values for a significance level of 0.05 (two-tailed) can be calculated through the z-table and are approximately -1.96 and 1.96.

Step 4: Result

Since the absolute value of the test statistic (2.04) is greater than the critical value (1.96), we reject the null hypothesis. And conclude that, there is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL

Limitations of Hypothesis Testing

• Although a useful technique, hypothesis testing does not offer a comprehensive grasp of the topic being studied. Without fully reflecting the intricacy or whole context of the phenomena, it concentrates on certain hypotheses and statistical significance.
• The accuracy of hypothesis testing results is contingent on the quality of available data and the appropriateness of statistical methods used. Inaccurate data or poorly formulated hypotheses can lead to incorrect conclusions.
• Relying solely on hypothesis testing may cause analysts to overlook significant patterns or relationships in the data that are not captured by the specific hypotheses being tested. This limitation underscores the importance of complimenting hypothesis testing with other analytical approaches.

Hypothesis testing stands as a cornerstone in statistical analysis, enabling data scientists to navigate uncertainties and draw credible inferences from sample data. By systematically defining null and alternative hypotheses, choosing significance levels, and leveraging statistical tests, researchers can assess the validity of their assumptions. The article also elucidates the critical distinction between Type I and Type II errors, providing a comprehensive understanding of the nuanced decision-making process inherent in hypothesis testing. The real-life example of testing a new drug’s effect on blood pressure using a paired T-test showcases the practical application of these principles, underscoring the importance of statistical rigor in data-driven decision-making.

Frequently Asked Questions (FAQs)

1. what are the 3 types of hypothesis test.

There are three types of hypothesis tests: right-tailed, left-tailed, and two-tailed. Right-tailed tests assess if a parameter is greater, left-tailed if lesser. Two-tailed tests check for non-directional differences, greater or lesser.

2.What are the 4 components of hypothesis testing?

Null Hypothesis ( ): No effect or difference exists. Alternative Hypothesis ( ): An effect or difference exists. Significance Level ( ): Risk of rejecting null hypothesis when it’s true (Type I error). Test Statistic: Numerical value representing observed evidence against null hypothesis.

3.What is hypothesis testing in ML?

Statistical method to evaluate the performance and validity of machine learning models. Tests specific hypotheses about model behavior, like whether features influence predictions or if a model generalizes well to unseen data.

4.What is the difference between Pytest and hypothesis in Python?

Pytest purposes general testing framework for Python code while Hypothesis is a Property-based testing framework for Python, focusing on generating test cases based on specified properties of the code.

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How To Test A Hypothesis In Biology

Table of Contents:

Hypothesis testing . One of the main goals of statistical hypothesis testing is to estimate the P value, which is the probability of obtaining the observed results, or something more extreme, if the null hypothesis were true. If the observed results are unlikely under the null hypothesis, your reject the null hypothesis. Alternatives to this “frequentist” approach to statistics include Bayesian statistics and estimation of effect sizes and confidence intervals.

A Bayesian would insist that you put in numbers just how likely you think the null hypothesis and various values of the alternative hypothesis are, before you do the experiment, and I’m not sure how that is supposed to work in practice for most experimental biology. But the general concept is a valuable one: as Carl Sagan summarized it, “Extraordinary claims require extraordinary evidence. “

• Introduction
• Biological vs. statistical null hypotheses
• Testing the null hypothesis
• Reporting your results
• Bayesian statistics
• Recommendations

Video advice: Hypotheses & Hypothesis tests

Hypothesis and Hypothesis Testing

There are different ways of doing statistics. The technique used by the vast majority of biologists, and the technique that most of this handbook describes, is sometimes called “frequentist” or “classical” statistics. It involves testing a null hypothesis by comparing the data you observe in your experiment with the predictions of a null hypothesis. You estimate what the probability would be of obtaining the observed results, or something more extreme, if the null hypothesis were true. If this estimated probability (the P value) is small enough (below the significance value), then you conclude that it is unlikely that the null hypothesis is true; you reject the null hypothesis and accept an alternative hypothesis.

MasteringBiology Ch. 1 Hypothesis Testing & Experimental Design Flashcards – Start studying MasteringBiology Ch. 1 Hypothesis Testing & Experimental Design. Learn vocabulary, terms, and more with flashcards, games, and other study tools.

Hypothesis Testing

Experiment:Purchase 10 tomato plants of the same type from the same nursery. Pick plants that are similar in size and age. Divide the plants into two groups of 5. Apply Brand A to the first group and Brand B to the second group according to the instructions on the packages. After 10 weeks, count the number of tomatoes on each plant.

Question – Biologists study the living world by posing questions about it and seeking science-based responses. This approach is common to other sciences as well and is often referred to as the scientific method. The scientific process was used even in ancient times, but it was first documented by England’s Sir Francis Bacon (1561–1626) (Figure 1), who set up inductive methods for scientific inquiry. The scientific method is not exclusively used by biologists but can be applied to almost anything as a logical problem solving method.

Hypothesis Generation in BiologyA Science Teaching Challenge & Potential Solution

© 2015 by the Regents of the University of California. All rights reserved. Please direct all requests for permission to photocopy or reproduce article content through the University of California Press’s Reprints and Permissions web page, ucpress. edu/journals. php? p=reprints. 2015.

• Generalizing & Explanatory Hypotheses
• Abduction, Deduction, & Induction
• Solving the Problem of “Hypothesis” in the Science Classroom

There Is a Problem: Data from the Field

Helping students understand and generate appropriate hypotheses and test their subsequent predictions – in science in general and biology in particular – should be at the core of teaching the nature of science. However, there is much confusion among students and teachers about the difference between hypotheses and predictions. Here, I present evidence of the problem and describe steps that scientists actually follow when employing scientific reasoning strategies. This is followed by a proposed solution for helping students effectively explore this important aspect of the nature of science.

2.2: Standard Statistical Hypothesis Testing

Standard hypothesis testing approaches focus almost entirely on rejecting null hypotheses. In the framework (usually referred to as the frequentist approach to statistics) one first defines a null hypothesis. This null hypothesis represents your expectation if some pattern, such as a difference among groups, is not present, or if some process of interest were not occurring. For example, perhaps you are interested in comparing the mean body size of two species of lizards, an anole and a gecko. Our null hypothesis would be that the two species do not differ in body size. The alternative, which one can conclude by rejecting that null hypothesis, is that one species is larger than the other. Another example might involve investigating two variables, like body size and leg length, across a set of lizard species1. Here the null hypothesis would be that there is no relationship between body size and leg length. The alternative hypothesis, which again represents the situation where the phenomenon of interest is actually occurring, is that there is a relationship with body size and leg length. For frequentist approaches, the alternative hypothesis is always the negation of the null hypothesis; as you will see below, other approaches allow one to compare the fit of a set of models without this restriction and choose the best amongst them.

I’ll cover a few examples from the frequentist approach within this book, mainly when discussing conventional methods like phylogenetic independent contrasts (PICs). Also, among the model selection approaches used frequently within this book, likelihood ratio tests, depend on the standard frequentist set-track of null and alternative ideas.

Video advice: Scientific Method – Test Your Hypothesis

This video will walk students through how to set up a controlled experiment and explain why controlled experiments are important.

Standard hypothesis testing approaches focus almost entirely on rejecting null hypotheses. In the framework (usually referred to as the frequentist approach to statistics) one first defines a null hypothesis. This null hypothesis represents your expectation if some pattern, such as a difference among groups, is not present, or if some process of interest were not occurring. For example, perhaps you are interested in comparing the mean body size of two species of lizards, an anole and a gecko. Our null hypothesis would be that the two species do not differ in body size. The alternative, which one can conclude by rejecting that null hypothesis, is that one species is larger than the other. Another example might involve investigating two variables, like body size and leg length, across a set of lizard species1. Here the null hypothesis would be that there is no relationship between body size and leg length. The alternative hypothesis, which again represents the situation where the phenomenon of interest is actually occurring, is that there is a relationship with body size and leg length.

The previous two chapters introduced methods for organizing and summarizing sample data, and using sample statistics to estimate population parameters. This chapter introduces the next major topic of inferential statistics: hypothesis testing.

Let’s answer this question using the p-value approach. Remember, for a two-sided alternative hypothesis (“not equal”), the p-value is two times the area of the test statistic. The test statistic is -1. 81 and we want to find the area to the left of -1. 81 from the standard normal table.

• A Two-sided Test
• A Right-sided Test
• A Left-sided Test

For example, past research tells us that the average life span for a hummingbird is about four years. You have been studying the hummingbirds in the southeastern United States and find a sample mean lifespan of 4. 8 years. Should you reject the known or accepted information in favor of your results? How confident are you in your estimate? At what point would you say that there is enough evidence to reject the known information and support your alternative claim? How far from the known mean of four years can the sample mean be before we reject the idea that the average lifespan of a hummingbird is four years?

what type of experiment is one way to test a hypothesis

What is Hypothesis Testing? Hypothesis testing in statistics is a way for you to test the results of a survey or experiment to see if you have meaningful.

With many true experiments, the investigator is attempting to determine a causal relationship between variables, by manipulating a completely independent variable to evaluate the result upon dependent variables. Within the simplest kind of experiment, the investigator is attempting to demonstrate when one event occurs, a particular outcome happens.

Contents1 What Type Of Experiment Is One Way To Test A Hypothesis? 2 What is test the hypothesis with an experiment? 3 What are the ways to test a hypothesis? 4 How do you test a hypothesis in scientific method? 5 How do you test the hypothesis in an experiment? 6 Can an experiment prove a hypothesis? 7 What are 4 ways to test a hypothesis? 8 How are hypotheses tested quizlet? 9 How research uses hypothesis testing? 10 What is experiment scientific method? 11 What is the hypothesis in scientific method? 12 What are types of hypothesis? 13 What are two ways to test a hypothesis? 14 Do all experiments have a hypothesis? 15 How many experiments are needed to prove a hypothesis is correct? 16 How do you test a hypothesis in psychology? 17 What are the 4 main components of a good experiment? 18 Which of these are types of hypothesis test? 19 Why hypothesis testing is performed? 20 What is known in statistical hypothesis testing? 21 What is a systematic way of testing a hypothesis? 22 What are quantitative hypothesis techniques?

Null hypothesis

All about null hypothesis, definition of null hypothesis, how to develop null hypothesis, examples of null hypothesis, validation of null hypothesis.

A hypothesis is understood to be an idea or perhaps an assumption that is dependant on insufficient evidence. It requires and needs more experiments and testing for confirmation. There’s two options that by doing more experiments and testing, a hypothesis could be false or true. This means it may either prove wrong or true (Blackwelder, 1982).

A null hypothesis is an assumption or proposition where an observed difference between two samples of a statistical population is purely accidental and not due to systematic causes. It is the hypothesis to be investigated through statistical hypothesis testing so that when refuted indicates that the alternative hypothesis is true. Thus, a null hypothesis is a hypothesis that is valid or presumed true until invalidated by a statistical test. And so, one cannot say that a null hypothesis is ‘’accepted’’ rather it “cannot be rejected” as it remains to be true based on the statistical evidence supporting it. Conversely, a null hypothesis that is refuted can be said to have been“rejected”. Word origin: coined by the English geneticist and statistician Ronald Fisher. Symbol: H0.

Experiments and Hypotheses

Now we’ll focus on the methods of scientific inquiry. Science often involves making observations and developing hypotheses. Experiments and further observations are often used to test the hypotheses.

Within the Try It below, you are able to practice recognizing scientific ideas. While you consider each statement, attempt to think like a researcher would: can one test this hypothesis with observations or experiments? May be the statement falsifiable? If the solution to either of those questions is “no,” the statement isn’t a valid scientific hypothesis.

• Learning Outcomes
• Practice Questions
• Practice Question

Experimental Design

A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always. For example, a scientist studying the mating behaviors of ladybugs might begin with detailed observations of ladybugs mating in their natural habitats. While this research may not be experimental, it is scientific: it involves careful and verifiable observation of the natural world. The same scientist might then treat some of the ladybugs with a hormone hypothesized to trigger mating and observe whether these ladybugs mated sooner or more often than untreated ones. This would qualify as an experiment because the scientist is now making a change in the system and observing the effects.

Video advice: Hypothesis testing. Null vs alternative

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How do you test your hypothesis?

There are 5 main steps in hypothesis testing:

• State your research hypothesis as a null (Ho) and alternate (Ha) hypothesis.
• Collect data in a way designed to test the hypothesis.
• Perform an appropriate statistical test.
• Decide whether to reject or fail to reject your null hypothesis.

What is an example of a hypothesis in biology?

Here are some examples of hypothesis statements: If garlic repels fleas, then a dog that is given garlic every day will not get fleas . If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities. If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

What are the 4 steps to test a hypothesis?

Step 1: State the hypotheses. Step 2: Set the criteria for a decision. Step 3: Compute the test statistic. Step 4: Make a decision .

How do you test a hypothesis in epidemiology?

There are two approaches to evaluating hypotheses: comparison of the hypotheses with the established facts and analytic epidemiology , which allows testing hypotheses. A comparison with established facts is useful when the evidence is so strong that the hypothesis does not need to be tested.

What is an example of hypothesis testing?

The main purpose of statistics is to test a hypothesis. For example, you might run an experiment and find that a certain drug is effective at treating headaches . But if you can't repeat that experiment, no one will take your results seriously.

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